شناسایی لاین های بازگرداننده باروری با استفاده از نشانگرهای STS در ارقام مختلف برنج ایران

In order to identify restorer lines in hybrid rice system, sixty lines and cultivars were studied. Two STS makers of RG140 that is linked to Rf3 locus on chromosome 1 and S10019 that is linked to Rf4 locus on chromosome 10 were used to screen restorer line .Then, after digestion with two restriction-enzymes (EcoRI and FnudII), the results showed that 27 lines had Rf3 gene, 3 lines had Rf4 gene and 20 lines had both fertility restorer genes (Rf3 and Rf4) with more than 85 percent fertility based on phenotypic test. Therefor these lines can be used as restorer lines in hybrid rice seed production. However the other lines with less than 85 percent field fertility can not be used as restorers.